HPLC Column, NP Amino, Wide Pore, 3um, 4.0mm ID x 250mm Length, 300A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent NP Amino™ Wide Pore is a normal-phase and HILIC stationary phase designed for the retention and separation of larger, highly polar compounds. Manufactured using a three-carbon propyl ligand with a terminal amino functional group bonded to ultra-high purity silica, this 300 Å wide pore stationary phase provides enhanced accessibility for larger analytes while maintaining the selectivity associated with USP L8 chemistry. It is an excellent choice as an alternative in USP methods requiring L8 functionality and for method development involving larger polar molecules.
68340-25P-4-3M is an HPLC column packed with 3 µm NP Amino™ Wide Pore particles featuring a 300 Å pore size in a 4.0 mm ID x 250 mm format. The 250 mm length provides maximum chromatographic resolution and peak capacity within the NP Amino product family, while the wide pore structure improves mass transfer and accessibility for larger analytes. The 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.
This column is particularly useful for complex sugars, larger compounds containing carboxylic acid groups, and other highly polar analytes analyzed using Normal Phase or HILIC chromatography. The amino functionality provides strong retention for polar molecules, while the 300 Å pore structure accommodates larger analytes that may be restricted on traditional 100 Å materials. As an unmodified silica bonded amino phase, this column is also suitable for HILIC applications. While Normal Phase chromatography is not commonly coupled with mass spectrometry, HILIC methods are frequently used with LC-MS systems for polar compound analysis.
Key Benefits
NP Amino™ Wide Pore stationary phase
USP L8 equivalent chemistry
Amino bonded phase
Ultra-high purity silica support
300 Å wide pore design
Optimized for larger molecules
Suitable for Normal Phase chromatography
Suitable for HILIC applications
3 µm particle size
Maximum chromatographic resolution
Typical Applications
USP L8 methods
Complex sugar analysis
Carbohydrate analysis
Organic acid analysis
Polar compound analysis
HILIC methods
Normal Phase chromatography
Food and beverage analysis
Pharmaceutical analysis
Biomolecule analysis
Specifications
Product Type: HPLC Column
Stationary Phase: NP Amino™ Wide Pore
USP Classification: L8
Chromatography Modes: Normal Phase and HILIC
Bonded Phase: Amino
Endcapping: Not End Capped
Particle Size: 3 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
Please review the Special Order Item Information for complete details.
For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.
Important Notes
Designed for larger polar compounds and complex carbohydrates
300 Å wide pores improve accessibility for larger analytes
Suitable for both Normal Phase and HILIC chromatography
As an unmodified silica bonded amino phase, this column is suitable for HILIC applications
The 250 mm length provides maximum resolution and peak capacity for demanding separations
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
Packed and shipped in Normal Phase solvents
Provides an excellent alternative for USP L8 methods involving larger analytes
Normal Phase chromatography is not commonly coupled to mass spectrometry, whereas HILIC methods are frequently used with LC-MS detection
Particularly useful for complex separations requiring maximum chromatographic resolution
This is a Special Order Item